Mixer Mill (Net weight – 275 kg )

The Mixer Mill (Net weight – 275 kg) is a true multipurpose mill designed for dry, wet and cryogenic grinding of small volumes up to 2 x 20 ml. It mixes and homogenizes powders and suspensions with a frequency of 30 Hz within seconds – unbeatably fast and easy to operate.

THE MOST VERSATILE ALL-ROUNDER OF BALL MILLS

• Max. speed 30 Hz

• Horizontal oscillation causes strong impact effects for effective sample processing

• Up to 8 mm feed size and 5 °m final fineness

• 2 grinding stations for jars of min 2 ml and max 50 ml, adapter for 10 x 2 ml single use vials and 4 x 50 ml conical centrifugation tubes

• Steel jars can be precooled manually in liquid nitrogen

• Calibrated speed and time, small benchtop model, storable SOPs and cycle programs, 7 different jar materials

SOLUTIONS FOR BIOLOGICAL APPLICATIONS AND CELL DISRUPTION

• Mixer mills are frequently used for homogenizing biological samples. The so-called bead beating with small glass beads is an established method for cell disruption of yeasts, microalgae or bacteria. The sample is only moderately warmed in the process which can be reduced to a minimum by pre-cooling.

• Mixer Mill (Net weight – 275 kg) allows for efficient cell disruption of up to 240 ml cell suspension for DNA/RNA and protein extraction. For accurate diagnosis of infections, it is possible to isolate intact bacteria from tissue in 8 x 30 ml bottles or 10 x 5 ml vials by using adapters.

• Mixer Mill (Net weight – 275 kg) can be operated with a range of adapters for single-use vials with the following capacities : 20 x 0.2 ml / 20 x 1.5 or 2 ml / 10 x 5 ml / 8 x 30 ml / 8 x 50 ml

For the pulverization of 25 to 30 g plant material, such as cannabis flower, conical centrifuge tubes are best suited. Up to 8 tissue samples, like fresh liver in buffer solution, can also be homogenized in these 50 ml tubes using steel or zirconium oxide balls. To keep the mechanical stress on the vials as low as possible, a reduced frequency and a high filling level, e. g. with buffer and sample, are recommended.

FUNCTIONAL PRINCIPLE

The grinding jars of the Mixer Mill (Net weight – 275 kg) perform radial oscillations in a horizontal position. The inertia of the grinding balls causes them to impact with high energy on the sample material at the rounded ends of the jars and pulverize it. Also, the movement of the jars combined with the movement of the balls result in the intensive mixing of the sample.

The degree of mixing can be increased even further by using several smaller balls. If several small balls are used (e.g. glass beads) then, for example, biological cells can be disrupted. The large frictional impact effects between the beads ensure effective cell disruption.

Applications :

• size reduction, mixing, homogenization, cell disruption, cryogenic grinding, mechanochemistry

Field of application :

• agriculture, biology, chemistry / plastics, construction materials, engineering / electronics, environment / recycling, food, geology / metallurgy, glass / ceramics, medicine / pharmaceuticals

Feed material :

• hard, medium-hard, soft, brittle, elastic, fibrous

Size reduction principle :

• impact, friction

Material feed size :

• <= 8 mm

Final fineness :

• ~ 5 °m

Batch size / feed quantity :

• max. 2 x 20 ml

No. of grinding stations :

• 2

Vibrational frequency :

• 3 - 30 Hz (180 - 1800 min-1)

Typical mean grinding time :

• 30 s - 2 min

Max. grindig time :

• 99 h

Dry grinding :

• yes

Wet grinding :

• yes

Cryogenic grinding :

• yes

Cell disruption with reaction vials :

• yes, up to 20 x 2.0 ml

Self-centering clamping device :

• yes

Type of grinding jars :

• screw top design

Material of grinding tools :

• hardened steel, stainless steel, tungsten carbide, agate, zirconium oxide, PTFE, PMMA

Grinding jar sizes :

• 1.5 ml / 5 ml / 10 ml / 25 ml / 35 ml / 50ml

Setting of grinding time :

• digital, 10 s - 8 h

Storable SOPs :

• 12

Storable cycle programs :

• 6

Electrical supply data :

• 100-240 V, 50/60 Hz

Power connection :

• 1-phase

Protection code :

• IP 30

Power consumption :

• 165W

W x H x D closed :

• 385 x 350 x 470 mm

Net weight :

• ~ 27,5 kg

Standards :

• CE