Langendorff and Working Heart Systems for Isolated Mini Pig or Small Domestic Pig Heart

The tabletop isolated heart perfusion system is designed for small pig hearts with a body weight of up to approximately 20 kg and can also be used for rabbit or mini pig models. The system provides stable perfusion and physiological conditions for longer experimental recordings with reduced artifacts. Its modular design allows operation in retrograde Langendorff perfusion or real ejecting working heart mode and can be expanded according to research requirements. The system supports hemodynamic, ischemia-reperfusion, cardioplegia, electrophysiology and cardiovascular research applications. Manufactured by Atico Export for reliable performance in demanding physiological research, experimental and scientific laboratories.

Advanced System Design :

• Designed for increased flow requirements of larger animal hearts
• Supports retrograde Langendorff perfusion under constant pressure
• Supports retrograde Langendorff perfusion under constant flow
• Supports real ejecting working heart operation
• Suitable for hemodynamic and ischemia-reperfusion studies
• Compatible with saline, erythrocyte-containing perfusion solutions and heparinized blood
• Integrated aortic block mounted on the heart chamber cover
• Working heart configuration includes a preload reservoir
• Large Windkessel functions as a bubble trap and damping reservoir
• Mimics aortic compliance during ejecting working heart perfusion
• Short connecting tubing helps reduce cooling and system-generated flow resistance
• Adjustable aortic pressure or afterload pressure
• Adjustable preload pressure in working heart mode
• Membrane system helps provide pressure waves that closely represent physiological conditions
• Large heart chamber acts as a reservoir to minimize perfusion solution volume
• Membrane oxygenator can be used for oxygenation of erythrocyte-containing perfusate
• Interchangeable aortic and atrial cannulae available for working heart configuration
• Electrically driven lift allows vertical adjustment of the platform
• Heated jacketed heart chamber supports improved temperature conditions
• Accommodates hearts with aorta diameters from 5 to 12 mm
• Retrograde perfusion pressure up to 300 mmHg
• Retrograde perfusion flow up to 500 ml/min
• Working heart aortic flow up to 1,500 ml/min

Applications :

• Study of myogenic autoregulation with ultrasonic flow measurement
• Testing of inotropic substances
• Testing of lusitropic substances
• Testing of vasoactive substances
• Cardiac rhythm studies
• Ischemia and hypoxia studies
• Refractory period studies
• Ischemia-reperfusion injury studies
• Cardioplegia studies
• Cardiac preconditioning studies
• Cardiovascular screening
• Electrophysiology studies
• Phenotyping of transgenic animals
• Drug compound screening
• Toxicology studies
• Biochemical testing
• Heart transplantation models
• Hemodynamic studies
• Cardiac flow studies
• Left ventricular pressure measurement
• Pressure-volume relationship studies

Working Heart Applications :

• Real-time measurement of aortic and atrial flow
• Intracardial left ventricular pressure measurement
• Pressure-volume measurement
• High atrial pressure-induced disease state simulation

Langendorff Mode :

• Low flow resistance and low dead space volume
• Helps minimize perfusion artifacts
• Compact configuration without high water columns
• Suitable for hearts requiring perfusion pressure up to 300 mmHg
• Supports constant pressure or constant flow operation
• Integrated aortic block
• Bubble trap positioned above the aortic cannula
• Optional integrated flow probe for real-time flow measurement
• Integrated stopcock for controlling perfusion flow and simulating ischemic conditions
• Drug injection pathway integrated into the aortic perfusate stream
• Minimizes temperature and oxygen loss
• Can be upgraded to working heart operation
• Modular configuration supports additional applications
• Dedicated electrodes and holders for precise measurements
• Electrodes and catheters positioned around the heart for convenient access

Working Heart Mode :

• Includes Langendorff mode features
• Optimized atrial cannulation conditions
• Easy switching between Langendorff and working heart modes
• Physiological flow resistance and minimal dead space volume
• Short atrial filling time for improved ventricular filling
• Built-in port for catheter transducer insertion
• Supports left ventricular pressure and pressure-volume measurement
• Mimics physiological atrial and arterial pressure and flow patterns
• Compact aortic block
• Membrane afterload system and compliance chamber for physiological afterload simulation
• Helps reduce potential damage to heart valves associated with water column systems
• Optional integrated flow probe for real-time aortic flow measurement
• Constant preload system
• Mimics physiological atrial pressure patterns
• Helps reduce stress on the mitral valve
• Preload pressure remains independent of atrial flow
• Optional real-time coronary flow measurement
• Coronary flow can be measured through pulmonary artery cannulation

Measured Signals :

Isovolumetric Left Ventricular Pressure :

• Measured using the balloon method

Aortic Pressure :

• Perfusion pressure measurement

Coronary Flow :

• Indirect or direct flow measurement options available

Calculated Parameters :

• dLVP/dt
• Maximum dLVP/dt
• Minimum dLVP/dt
• Contractility index
• Systolic left ventricular pressure
• Diastolic left ventricular pressure
• Heart rate
• Mean perfusion pressure
• Mean perfusion flow
• Coronary resistance

Core System :

• Tabletop stand
• Aortic block with Windkessel and bubble trap
• Adjustable artificial flow resistance
• Pressure gauge and manometer
• Jacketed heart chamber
• Electrical lift
• Filter
• Tubing and connections
• Set of aortic cannulae for aorta diameters from 5 to 12 mm
• Expandable to working heart configuration

Working Heart Option :

• Left atrium cannulating system
• Preload reservoir with 0.3 L capacity
• Movable atrium connection adapter
• Set of left atrial cannulae for aorta diameters from 5 to 12 mm
• Supports aortic flow up to 1.5 L/min

Additional Components :

Oxygenator :

• Required for oxygenating and warming blood or perfusion solution
• Suitable clinical oxygenator systems can be used

Amplifier :

• Required for pressure and flow measurements
• Supports ECG and MAP measurement applications

Data Acquisition System :

• Provides real-time evaluation of multiple signals and cardiovascular parameters
• Supports recording and analysis of experimental data
• Can support flow-proportional drug addition using a syringe pump

Specialized Applications & Upgrades :

• Working heart configuration
• Cardioplegia studies
• Direct coronary and aortic flow measurement
• Intracardial left ventricular pressure measurement
• Pressure-volume loop measurement
• Increased preload pressure
• Perfusate oxygenation
• Temperature measurement
• Perfusion solution monitoring
• Coronary effluent collection
• Drug addition
• Perfusate filtration
• Cardiac pacing
• Single-lead ECG and MAP measurement
• Multi-lead ECG and MAP measurement