Advanced System Design :
- Designed for isolated heart perfusion of rabbit, adult guinea pig and adult rat hearts
- Supports flow rates up to 500 mL/min
- Provides stable perfusion conditions for longer experimental recordings
- Combines the advantages of ex vivo preparation with in situ-like perfusion features
- Compact design without high water columns
- Uses pressure feedback for constant pressure perfusion
- Supports constant pressure and constant flow perfusion
- Allows switching between different perfusion modes and perfusion solutions
- Aortic block mounted on a compact acrylic stand
- Starling resistor can provide constant pressure or constant flow operation depending on pressure settings
Measured Signals :
- Isovolumetric left ventricular pressure with balloon
- Aortic perfusion pressure
- Coronary flow
Calculated Parameters :
- dLVP/dt
- Maximum dLVP/dt
- Minimum dLVP/dt
- Contractility index
- Systolic and diastolic left ventricular pressure
- Heart rate
- Mean perfusion pressure
- Mean perfusion flow
- Coronary resistance
Core System Includes :
- Base unit
- Thermostatic circulator with 5 L bath
- Peristaltic pump
- Instrument case
- Transducer amplifier module
- Transducer amplifier module for pressure measurement
- Two pressure transducers
- Transducer holder
Base Unit Includes :
- Acrylic stand
- Aortic block with aorta link unit and Windkessel chamber
- Heat exchanger
- Jacketed heart chamber
- Needle valve for adjusting gas flow
- Holder for perfusion pressure transducer
- Tubing
- Aortic cannula and buffer reservoir must be added separately
Additional Requirements for a Functional Langendorff Unit :
- Perfusion pressure controller
- Species-specific additions
- Application-appropriate heart chamber
- Species-specific cannulae, buffer reservoirs and balloons
- Suitable data acquisition system
Specialized Applications & Upgrades :
Working Heart Upgrade :
- Core system can be upgraded to a working ejecting heart configuration
- Supports aortic flows up to 500 mL/min
- Suitable for researchers planning future working heart experiments
- Working heart configuration requires the appropriate working heart upgrade and species-specific additions
Biventricular Working Heart Upgrade :
- Allows both sides of the heart to eject fluid under controlled conditions
- Flow resistance and compliance components simulate physiological afterload
Coronary Flow Measurement :
- Indirect measurement by controlling the speed of a peristaltic pump
- Direct measurement using an ultrasonic transit-time flow measurement technique
Coronary Effluent Collection :
- Supports collection of coronary effluent for metabolic studies and further analysis
- Enables continuous measurement of pO2, pH and pCO2 when appropriate components are used
Perfusate Oxygenation :
- Suitable for oxygenation of buffer solutions containing albumin, fatty acids, washed erythrocytes or other foaming additives
Perfusate Filtration :
- Provides filtration of recirculated perfusate
Temperature Measurement :
- Enables measurement of perfusate temperature
- Particularly useful for temperature-controlled studies such as cooling or transplantation studies
Perfusion Solution Monitoring :
- Continuous or intermittent measurement of pO2
- Continuous or intermittent measurement of pH
- Continuous or intermittent measurement of pCO2
Drug Addition :
- Accurate drug addition using a syringe pump
- Supports flow-controlled drug addition when flow is measured or calculated and a specific drug-to-flow ratio is required
Pacing Option :
- Provides pacing for functional Langendorff and Working Heart systems
- Suitable for small rodent hearts including mouse, rat and guinea pig
Single-Lead ECG and MAP Measurement :
- Supports measurement of one ECG lead in combination with MAP electrodes
Multi-Lead ECG and MAP Measurement :
- Supports measurement of up to 12 ECG leads combined with up to 8 MAP electrodes
- Suitable for rat, guinea pig and rabbit hearts
