Advanced System Design :
- Provides ease-of-use and stability of measurements while allowing upgrades to advanced capabilities
- Uses safer pressure-control methods instead of high water columns
- Supports pressure control using a Starling resistor or feedback-based perfusion controller
- Fully enclosed aortic block within the water-jacketed upper and lower heart chamber
- Thermally stable precision-machined construction for precise temperature control
- Larger heart chamber suitable for ECG, MAP, LVP and pacing applications
Measured Signals :
- Isovolumetric left ventricular pressure (LVP) using the balloon method
- Aortic perfusion pressure
- Coronary flow
Calculated Parameters :
- dLVP/dt
- Maximum dLVP/dt
- Minimum dLVP/dt
- Contractility index
- Systolic LVP
- Diastolic LVP
- Heart rate
- Mean perfusion pressure
- Mean perfusion flow
- Coronary resistance
Core System Includes :
- Base unit for small rodent hearts up to 800 g
- Thermostatic circulator
- Peristaltic pump
- Transducer housing
- Transducer amplifier modules
- Pressure transducers
- Transducer holder
- Block clamp for mounting transducer onto rod
Base Unit Includes :
- Plexiglas stand
- Aortic block with aorta link unit
- Windkessel
- Heat exchanger
- Jacketed heart chamber
- Needle valve for adjusting gas flow
- Holder for perfusion pressure transducer
- Tubing
- Aortic cannula and buffer reservoir are required separately
Additional Requirements for a Functional Langendorff Unit :
- Perfusion pressure controller
- Species-specific additions
- Cannulae
- Buffer reservoirs
- Balloons
- Appropriate data acquisition system
Additional Components :
Data Acquisition System :
- Provides real-time evaluation of a wide range of signals and cardiovascular parameters
Thermostatic Circulator :
- Used to warm and maintain the temperature of the perfusate
Buffer Reservoirs :
- Used with a peristaltic pump to deliver warmed perfusate to the target organ
- Used to aerate the perfusate with oxygen and carbon dioxide
Working Heart Upgrade :
- Allows the Langendorff Core System to be upgraded to a fully ejecting working heart
- Supports aortic flows up to 60 ml/min
- Requires the working heart upgrade and a species-specific addition
- A Langendorff Working Heart-Ready configuration can be selected for future working heart applications
- Direct coronary flow measurement requires a suitable flowmeter and species-appropriate flow probe
- The standard perfusion controller used for Langendorff flow control cannot be used for working heart operation
Coronary Flow Measurement Option :
- Indirect measurement by controlling the pump speed of a peristaltic pump
- Direct measurement using ultrasound transit-time technology
Coronary Effluent Collection :
- Pulmonary artery cannulae
- Preparation dish
- Effluent funnel
- Collection of coronary effluent for metabolic studies and further analysis
- Supports continuous measurement of pO2, pH and pCO2
Perfusate Oxygenation Option :
- Provides oxygenation of buffer solution supplemented with albumin, fatty acids, washed erythrocytes or other foaming additives
Perfusate Filtration Option :
- Provides filtration of recirculated perfusate
Temperature Measurement Option :
- Measures perfusate temperature in isolated perfused organ systems
- Useful for specific temperature studies and special temperature protocols such as cooling or transplantation studies
Perfusion Solution Monitoring :
- Enables precise continuous or intermittent measurement of pO2, pH and pCO2 in liquid media or perfusate
Drug Addition Option :
- Supports controlled drug addition where flow is measured or calculated and the drug must be added in a specific ratio
Cell Isolation Upgrade :
- Supports cell isolation and cell extraction applications
- Designed for use with a functional working heart system
Pacing Option :
- Provides pacing for functional Langendorff and working heart systems
- Suitable for small rodent hearts including mouse, rat and guinea pig
Single-Lead ECG and MAP Measurement Option :
- Provides single-lead ECG and MAP measurement
- Combines one ECG lead with one MAP electrode
