Core System Options :
Langendorff Only Configuration :
- Species-specific addition for rat and guinea pig hearts or rabbit hearts
- Constant pressure/flow controller for perfusion pressure and flow control
- Heart chamber for cardiovascular studies or electrophysiology studies
Langendorff Working Heart-Ready Configuration :
- Species-specific addition for rat and guinea pig hearts or rabbit hearts
- Starling resistor for perfusion pressure control in Langendorff mode and afterload control in working heart mode
- Heart chamber for cardiovascular studies or electrophysiology studies
Perfusion Pressure Controller :
Constant Pressure/Flow Controller :
- Maintains perfusion at constant pressure or constant flow using a peristaltic pump
- Modulates pump-generated flow through a perfusion pressure feedback loop
- Provides accurate control of perfusion pressure or flow, including at very low flow rates
- Calculates flow rate from pump speed
- Allows operation in constant pressure or constant flow mode
- Flexible perfusion circuit setup for different experimental conditions
Measured Signals and Calculated Parameters :
- Indirect coronary flow measurement
- Coronary resistance calculation
Starling Resistor Perfusion Pressure Controller :
- Provides constant-pressure perfusion in Langendorff mode
- Functions as an afterload system in working heart mode
- Uses a membrane flow resistor, manometer and pressure syringe to create a pressure-controlled valve in the aortic block
- Mechanical pressure-control system that does not provide flow information or alter pump flow rate
- Pump flow is set above the required level, while only the flow producing the selected perfusion pressure is delivered to the heart
- Remaining flow exits through the valve and returns to the reservoir
- Direct coronary flow measurement is required when coronary flow data is needed
Measured Signals and Calculated Parameters :
- Coronary flow data through the species-specific flow measurement option
Includes :
- Aortic block base unit with flange-mounted adjustable flow resistance
- Pressure syringe with mounting bracket
- Manometer
Species-Specific Options :
Rat and Guinea Pig Hearts :
- Aortic cannula set for rat and guinea pig hearts, 3.0 mm and 2.5 mm OD
- Tubing, 2.06 mm ID, 12 pieces
- Tubing set for jacketed buffer reservoir with fluid line shutoff valves
- Jacketed reservoir for buffer solution with frit and bottom drain, 2 L
- Balloon kit including 10 balloons, size 5, 0.1 ml volume, spindle set and holder
Rabbit Hearts :
- Aortic cannula set for rabbit hearts, 3.0 mm, 4.0 mm and 5.0 mm OD
- Tubing, 2.79 mm ID, 12 pieces
- Tubing set for jacketed buffer reservoir with fluid line shutoff valves
- Jacketed reservoir for buffer solution with frit, 6 L
- Balloon kit including 5 balloons, size 12, 1.3 ml volume, and 5 balloons, size 13, 1.6 ml volume, with spindle set and holder
Heart Chamber Options :
- Heart chamber for cardiovascular studies
- Heart chamber for electrophysiology studies
- Larger heart chamber required when using multi-ECG or circular MAP rings
Configuration Considerations :
- Langendorff-only configuration can be modified to a Langendorff working heart-ready configuration in the future
- Working heart-ready configuration is suitable when future upgrading to working ejecting heart studies is planned
- Selection of species-specific components depends on the heart model being studied
- Species-specific cannulae and related components are selected according to the animal model
