Precision Cut Lung Slices Research Platform for Physiological Research Instrumentation

The Precision Cut Lung Slices (PCLS) Research Platform is designed for automated imaging and physiological studies of pulmonary tissues. It combines high-speed imaging, fluorescence microscopy and precision positioning for airway contractility, dose response, cilia beating and calcium imaging research. The platform supports studies using tissues derived from mice, rats, pigs and human lungs. Manufactured by Atico Export with quality-focused production and attention to engineering specifications for physiological research, experimental studies and scientific training.

• Designed for Precision-Cut Lung Slice (PCLS) imaging research
• Supports automated airway contractility studies
• Suitable for dose response studies
• Supports cilia beating analysis
• Suitable for calcium imaging studies
• Applicable to pulmonary tissues derived from mice, rats, pigs and human lungs
• Combines high-speed imaging, 3-channel fluorescent microscopy and precision 3-axis positioning
• Provides automated imaging and physiological research capabilities

Techniques & Measurements :

• Imaging
• Lumen area detection
• Cilia beating frequency
• Dosing
• Airway contractility
• Dose response analysis
• Calcium imaging

Specifications :

Weight :

• Microscope: 50 lbs
• Doser: 35 lbs

Automated Objectives :

• Objective switcher with 3 objective slots
• 4× objective: 3.5 mm FOV
• 10× objective: 1.4 mm FOV
• 20× objective: 0.7 mm FOV

Camera :

• 5 MP monochrome camera
• Resolution: 2448 × 2048
• Pixel size: 3.45 μm
• High-speed camera: 75 FPS with full field of view

XY & Z Stage :

• XY resolution: 1 μm
• XY total travel: 120 mm
• Z resolution: 150 nm
• Z total travel: 18 mm

Brightfield & Fluorescence :

• Software-controlled lighting
• Three fluorescence channels through solid-state lighting
• DAPI
• GFP
• TRITC / TDTomato / Orange #1 FISH

Sample :

• Standard 6-well plates
• Well plate presence detection
• Well volume used: 5.5 ml

Dosing :

• Heating up to 45°C
• Dosing from one of eight 500 ml bottles
• Doser nozzle travel up/down: 0.65 inch (16.6 mm)
• Overflow, nozzle presence and nozzle tilt detection sensors
• Media exchange for a dose in less than 20 seconds
• Pump flow rate: 2–3 ml/s
• Doser dead volume: less than 5 ml
• Serial dosing: 1 well at a time
• Serial dosing imaging: every 20 seconds or faster depending on the number of airways
• Parallel dosing: 3 wells in parallel
• Parallel dosing imaging: every minute depending on the number of airways
• Up to 4 airways per slice

Slicer :

• Precision lung tissue slicer available as an optional component

• Automated operation
• Intuitive and easy to use
• Reproducible experimental results
• High-speed imaging
• 3-channel fluorescent microscopy
• Precision 3-axis positioning
• High-quality image acquisition
• Automated airway analysis
• Suitable for pulmonary tissue research